Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

RNA-seq analysis of Tetrahymena thermophila

ABSTRACT: We present a comprehensive transcriptome of ciliate T. thermophila using the Illumina RNA-seq platform. The data was generated from the six mRNA samples of growth, starvation and conjugation of Tetrahymena. Despite an AT rich genome, there are about 124.6 million reads mapped to T. thermophila genome. Using these mapped reads, we have significantly improved the previous genome annotation and investigated the gene expression. Besides, our result also provided a comprehensive understanding of the alternative splicing in T. thermophila, and suggested the existence of the regulated unproductive splicing and translation (RUST) in the single-celled eukaryote. RNA-seq for six samples of Tetrahymena growth, starvation and conjugation.

ORGANISM(S): Tetrahymena thermophila

SUBMITTER: Wei Miao 

PROVIDER: E-GEOD-27971 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Transcriptome analysis of the model protozoan, Tetrahymena thermophila, using Deep RNA sequencing.

Xiong Jie J   Lu Xingyi X   Zhou Zhemin Z   Chang Yue Y   Yuan Dongxia D   Tian Miao M   Zhou Zhigang Z   Wang Lei L   Fu Chengjie C   Orias Eduardo E   Miao Wei W  

PloS one 20120207 2

<h4>Background</h4>The ciliated protozoan Tetrahymena thermophila is a well-studied single-celled eukaryote model organism for cellular and molecular biology. However, the lack of extensive T. thermophila cDNA libraries or a large expressed sequence tag (EST) database limited the quality of the original genome annotation.<h4>Methodology/principal findings</h4>This RNA-seq study describes the first deep sequencing analysis of the T. thermophila transcriptome during the three major stages of the l  ...[more]