Uncoupling growth and product formation kinetics to design improved strains for recombinant protein production in escherichia coli

ABSTRACT: The ability to control the synthesis of products in the absence of cell division remains an attractive alternative for the optimization of microbial processes. If this could be achieved, it would dramatically expand the productivity of many microbial processes by increasing product synthesis in the absence of an increase in cell mass. In the present work we have looked at the factors involved in the design of a host where the fluxes would be channeled towards product formation rather than biomass synthesis. To identify the genes responsible for diverting the metabolic flux specifically towards product formation we have used for this study is quiescent-cell (Q-cell) expression system, in which a plasmid-encoded protein is expressed in nongrowing but metabolically active cells. These cells

ORGANISM(S): Escherichia coli

SUBMITTER: Krishna Mukherjee 

PROVIDER: E-GEOD-29486 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress