The inactive X chromosome adopts a unique three-dimensional conformation that is dependent on Xist RNA.

ABSTRACT: 3D-topology of DNA in the cell nucleus provides a level of transcription regulation beyond the sequence of the linear DNA. To study the relationship between transcriptional activity and spatial environment of a gene, we have used allele-specific 4C-technology to produce high-resolution topology maps of the active and inactive X-chromosomes in female cells. We found that loci on the active X form multiple long-range interactions, with spatial segregation of active and inactive chromatin. On the inactive X, silenced loci lack preferred interactions, suggesting a unique random organization inside the inactive territory. However, escapees, among which is Xist, are engaged in long-range contacts with each other, enabling identification of novel escapees. Deletion of Xist results in partial re-f

ORGANISM(S): Mus musculus

SUBMITTER: Erik Splinter 

PROVIDER: E-GEOD-29509 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress