RNA processing in Bacillus subtilis: Identification of targets of the essential RNase Y

ABSTRACT: RNA processing and degradation is initiated by endonucleolytic cleavage of the target RNAs. In many bacteria, this activity is performed by RNase E which is not present in Bacillus subtilis and other Gram-positive bacteria. Recently, the essential endoribonuclease RNase Y has been discovered in B. subtilis. This RNase is involved in the degradation of bulk mRNA suggesting a major role in mRNA metabolism. However, only a few targets of RNase Y have been identified so far. In order to assess the global impact of RNase Y, we compared the transcriptomes of strains expressing RNase Y or depleted for RNase Y. Our results indicate that processing by RNase Y results in accumulation of about 80 mRNAs. Some of these targets were substantially stabilized by RNase Y depletion, resulting in half-lives

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

SUBMITTER: Ulrike Mäder 

PROVIDER: E-GEOD-30430 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress