Unknown,Transcriptomics,Genomics,Proteomics

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Cryptococcal H99 cells grown in 8 conditions for capsule induction

ABSTRACT: To understand the relationship between gene expression and capsule formation, H99 cells were cultured overnight at 37ºC in the following eight conditions: low iron medium with or without both 500 mM ethylenediaminetetraacetic acid (EDTA) and 10 mM bathophenanthroline disulfonate (BPDS); phosphate-buffered saline (PBS) with or without 10% v/v fetal bovine serum; Dulbecco's Modified Eagle's Medium (from Sigma) in ambient air or 5% CO2; and Littman's medium with either 0.01 µg/ml or 1 µg/ml thiamine. All samples were hybridized against a common reference pool of total RNA. Three biological replicates were performed for each growth condition, with the exception of growth in DMEM.

ORGANISM(S): Cryptococcus neoformans

SUBMITTER: Brian Haynes

PROVIDER: E-GEOD-31911 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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