Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Cj1223c Mutant vs. Overexpressed, Time Course

ABSTRACT: The Cj1223c gene was cloned downstream of a strong promoter into the replicating plasmid pRY108, and was highly expressed in the wild type Campylobacter jejuni 81-176 strain (overexpressed). The Cj1223c gene was knocked out by allelic replacement in the 81-176 strain background (mutant). This mutant also carried an empty pRY108 vector. The Cj1223c overexpressing strain and the mutant were grown overnight in liquid broth, supplemented with kanamycin. The following morning, both cultures were diluted back to O.D.600=0.100, and allowed to continue to grow, shaking. At various time intervals (2, 6, 10, and 24 hours), samples of each culture were removed, combined with 1/10 stop solution, and the bacteria pelleted for RNA preparation. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Computed

ORGANISM(S): Campylobacter jejuni

SUBMITTER: Joanna Mackichan

PROVIDER: E-GEOD-3198 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The Campylobacter jejuni dccRS two-component system is required for optimal in vivo colonization but is dispensable for in vitro growth.

MacKichan Joanna K JK Gaynor Erin C EC Chang Christopher C Cawthraw Shaun S Newell Diane G DG Miller Jeff F JF Falkow Stanley S

Molecular microbiology 20041201 5

A Campylobacter jejuni two-component signal transduction system (TCSTS), designated dccR-dccS (diminished capacity to colonize; Cj1223c-Cj1222c), has been found to be important for in vivo colonization but dispensable for in vitro growth. A DeltadccR response regulator mutant generated using the virulent strain 81-176 background exhibited significantly reduced colonization of immunocompetent limited flora (I-LF) mice, severe combined immunodeficient limited flora (SCID-LF) mice, and 1-day-old ch ...[more]

PMID: 15554967

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Dataset Information

Cj1223c Mutant vs. Overexpressed, Time Course

Publications

The Campylobacter jejuni dccRS two-component system is required for optimal in vivo colonization but is dispensable for in vitro growth.

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