Transcription profiling of mouse sorted bone marrow samples to determine genes implicated in hematopoetic stem cell identity
Ontology highlight
ABSTRACT: Gene expression profiles of sorted bone marrow samples to determine genes implicated in hematopoetic stem cell identity. Experiment Overall Design: this experiment include 6 samples and 32 replicates
ORGANISM(S): Mus musculus
SUBMITTER: OGIC Info Ontario Genomics Innovation Centre (OGIC)
Project description:The pigmented portion of ciliary epithelium in the adult mammalian eye harbors mitotically quiescent retinal sphere cells, which are capable of self-renewal and differentiating into retinal neurons when assayed in vitro; however, very little is known about the molecular mechanism controlling the proliferation and differentiation of these adult retinal stem cells or their molecular resemblance to mutipotent stem/progenitor cells during early eye development. This experiment studies the gene expression of first passage and primary human and mouse retinal sphere cells. Experiment Overall Design: this experiment include 4 samples and 12 replicates
Project description:Mammary gland cells were isolated from 5-8 week old FVB mice and allowed to proliferate as undifferentiated mammospheres in the presence of growth factors (bFGF and EGF). Comparison of the gene expression profiles of undifferentiated mammospheres vs. mammospheres that were allowed to differentiate for 6 days by the removal of growth factors will help to identify genes that are implicated in the maintenance of the mammary gland stem cell compartment. Experiment Overall Design: this experiment include 2 samples and 12 replicates
Project description:Investigation of very early events required for initiating differentiation of pluripotent embryonal carcinoma cells - the effect of cell aggregation on gene expression. Experiment Overall Design: this experiment include 4 samples and 24 replicates
Project description:Study of differences in gene expression profiles in the mammary glands tumours of PEA3-null and wild-type PEA3 mice induced by the Her2/Neu transgene. Experiment Overall Design: this experiment include 3 samples and 22 replicates
Project description:Satellite cells play an important role in post-natal growth and regeneration of skeletal muscle. They can be defined as a population adult muscle stem cells based on their self renewal capability and ability to differentiate into skeletal muscle fibers. Functional Retinoblastoma protein (pRb) is essential for the process of skeletal muscle differentiation in satellite cell derived primary myoblasts. Furthermore, the biochemical function of pRb is largely associated with its ability to interact with chromatin modifying factors such as histone deacetylases (HDACs) and histone methyltransferases thus inhibiting transcription of target gene promoters. Hence, expression profiling of pRb null primary myoblasts and myotubes will provide a global picture of the downstream targets of pRb transcriptional regulation in relation to cell cycle control, apoptosis inhibition, and muscle differentiation. Experiment Overall Design: This experiment includes 4 samples with 3 replicates each on 2 platforms for a total of 24 Samples.
Project description:Comparison of gene expression in differentiating limb bud mesenchymal cells harvested at 0, 1 day and 3 days culture. Experiment Overall Design: this experiment include 3 samples and 27 replicates
Project description:Investigation of human hematopoietic stem cells gene expression patterns originating from different stages of ontogeny including fetal blood, cord blood, bone marrow, and mobilized peripheral blood in Lin-CD34+CD38- versus Lin-CD34+CD38+ populations. Experiment Overall Design: this experiment include 7 samples and 42 replicates
Project description:Comparison of NRA derived from SCA1+, CD45- MACS purified putative muscle derived stem cells vs. RNA derived from preplating of hind limb muscle cells. Experiment Overall Design: this experiment include 2 samples and 8 replicates
Project description:Muscle satellite cells are a self-renewing pool of stem cells that give rise to daughter myogenic precursor cells in adult skeletal muscle. Published and preliminary data indicated that MyoD and p53 genes are involved in satellite cell differentiation. We would like to know what downstream genes of both transcription factors are affected in satellite cell-derived myoblasts (MyoD-/-, p53 -/-). Experiment Overall Design: this experiment include 3 samples and 25 replicates