Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Scf-GFP+ cells from the bone marrow and whole bone marrow microarray

ABSTRACT: The HSC niche factor SCF is required for HSC maintenance. Using an Scf-GFP knockin mouse, we have identified a perivascular cell type in the bone marrow expressing high level of Scf. To characterize the novel Scf-GFP+ cells from the bone marrow, we performed microarray analysis on these cells. Total RNA were isolated from 3 independent, freshly aliquots of FACS sorted 5,000 SCF-GFP+ cells or whole bone marrow cells isolated from young adult mice. Purified RNA was amplified using the WT-OvationM-bM-^DM-" Pico RNA Amplification system (NuGEN Technologies). Sense strand cDNA was generated using WT-OvationM-bM-^DM-" Exon Module (NuGEN), then fragmented and labeled using the FL-OvationM-bM-^DM-" cDNA Biotin Module V2 (NuGEN). 2.5M-BM-5g of labeled cDNA were hybridized to Affymetrix Mouse Gene ST 1.0 microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Lei Ding

PROVIDER: E-GEOD-33158 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Endothelial and perivascular cells maintain haematopoietic stem cells.

Ding Lei L Saunders Thomas L TL Enikolopov Grigori G Morrison Sean J SJ

Nature 20120125 7382

Several cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources of these factors are undetermined. Stem cell factor (SCF; also known as KITL) is a key niche component that maintains HSCs. Here, using Scf(gfp) knock-in mice, we found that Scf was primarily expressed ...[more]

PMID: 22281595

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Dataset Information

Scf-GFP+ cells from the bone marrow and whole bone marrow microarray

Publications

Endothelial and perivascular cells maintain haematopoietic stem cells.

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