Gene expression profiles of bone marrow mesenchymal stem cells in pediatric patients with severe aplastic anemia

ABSTRACT: Pediatric severe aplastic anemia (PSAA) is a rare and life-threatening disease, which has been suggested to result from deficiency in essential cytokines or growth factors in bone marrow mesenchymal stem cells (MSCs). In this study, we examined expression profiles of the essential cytokines and growth factors, which were involved in proliferation and differentiation of MSC, in 5 PSAA patients using oligonucleotides microarrays. Following evaluation of gene expression patterns using cluster analysis and gene ontology classifications, fifteen potential genes that were cell proliferation- and cytokine-related and significantly down-regulated in PSAA were selected. The findings of the microarray analysis were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).

ORGANISM(S): Homo sapiens

SUBMITTER: Kuan-Chih Chow 

PROVIDER: E-GEOD-33812 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress