Comparative transcriptome analysis of Saccharomyces cerevisiae wild type and M-NM-^Tasc1 strain

ABSTRACT: Asc1p and its essential orthologues in higher eukaryotes, as e.g. RACK1 in mammals, are involved in several distinct cellular signaling processes, but the implications of a total deletion have never been assessed in a comprehensive genome-wide manner. This study reveals the major cellular processes affected in a Saccharomyces cerevisiae M-NM-^Tasc1 deletion background via de novo proteome and transcriptome analysis, as well as subsequent phenotypical characterizations. The deletion of ASC1 reduces iron-uptake and causes nitrosative stress, both known indicators for hypoxia, which manifests in a shift of energy metabolism from respiration to fermentation in the M-NM-^Tasc1 strain. The impact of Asc1p in cellular metabolism is further expressed by its regulative role in the MAP kinase signal

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Claudia Pommerenke 

PROVIDER: E-GEOD-34286 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress