Molecular mechanism underlying the regulatory specificity of a Drosophila homeodomain protein that specifies myoblast identity

ABSTRACT: A subfamily of Drosophila homeodomain (HD) transcription factors (TFs) controls the identities of individual muscle founder cells (FCs). However, the molecular mechanisms by which these TFs generate unique FC genetic programs remain unknown. To investigate this problem, we first applied genome-wide mRNA expression profiling to identify genes that are activated or repressed by the muscle HD TFs Slouch (Slou) and Muscle segment homeobox (Msh). Next, we used protein binding microarrays to define the sequences that are bound by Slou, Msh and other HD TFs having mesodermal expression. These studies revealed that a large class of HDs, including Slou and Msh, predominantly recognize TAAT core sequences but that each HD also binds to unique sites that deviate from this canonical motif. To better u

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Leila Shokri 

PROVIDER: E-GEOD-35380 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress