Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Diversity, Topographic Differentiation, and Positional Memory in Human Fibroblasts

ABSTRACT: A fundamental feature of the architecture and functional design of vertebrate animals is a stroma, composed of extracellular matrix and mesenchymal cells, which provides a structural scaffold and conduit for blood and lymphatic vessels, nerves, and leukocytes. Reciprocal interactions between mesenchymal and epithelial cells are known to play a critical role in orchestrating the development and morphogenesis of tissues and organs, but the roles played by specific stromal cells in controlling the design and function of tissues remain poorly understood. The principal cells of stromal tissue are called fibroblasts, a catch-all designation that belies their diversity. We characterized genome-wide patterns of gene expression in cultured fetal and adult human fibroblasts derived from skin at different anatomical sites. Fibroblasts from each site displayed distinct and characteristic transcriptional patterns, suggesting that fibroblasts at different locations in the body should be considered distinct differentiated cell types. Notablegroups of differentially expressed genes included some implicated in extracellular matrixsynthesis, lipid metabolism, and cell signaling pathways that control proliferation, cellmigration, and fate determination. Several genes implicated in genetic diseases werefound to be expressed in fibroblasts in an anatomic pattern that paralleled the phenotypicdefects. Finally, adult fibroblasts maintained key features of Hox gene expressionpatterns established during embryogenesis, suggesting that Hox genes may directtopographic differentiation and underlie the detailed positional memory in fibroblasts. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc

ORGANISM(S): Homo sapiens

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PROVIDER: E-GEOD-3551 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene expression signature of fibroblast serum response predicts human cancer progression: similarities between tumors and wounds.

Chang Howard Y HY Sneddon Julie B JB Alizadeh Ash A AA Sood Ruchira R West Rob B RB Montgomery Kelli K Chi Jen-Tsan JT van de Rijn Matt M Botstein David D Brown Patrick O PO

PLoS biology 20040113 2

Cancer invasion and metastasis have been likened to wound healing gone awry. Despite parallels in cellular behavior between cancer progression and wound healing, the molecular relationships between these two processes and their prognostic implications are unclear. In this study, based on gene expression profiles of fibroblasts from ten anatomic sites, we identify a stereotyped gene expression program in response to serum exposure that appears to reflect the multifaceted role of fibroblasts in wo ...[more]

PMID: 14737219

Publication: 1/2

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Dataset Information

Diversity, Topographic Differentiation, and Positional Memory in Human Fibroblasts

Publications

Gene expression signature of fibroblast serum response predicts human cancer progression: similarities between tumors and wounds.

Similar Datasets

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