Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Decoding human cytomegalovirus using ribosome profiling

ABSTRACT: The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like other complex viruses, our understanding of its protein coding potential is far from complete. Here, we use ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified several hundred previously unidentified open reading frames and confirmed a fraction by mass spectrometry. We found that regulated use of alternative transcript start sites plays a broad role in enabling tight temporal control of HCMV protein expression and allowing multiple distinct polypeptides to be generated from a single genomic locus. Our results reveal an unanticipated complexity to the HCMV coding capacity and illustrate the role of regulated changes in transcript start sites in generating this complexity. Ribosome profiling and mRNA-seq from 3 time points (5hr, 24hr, 72hr) along HCMV infection. The supplementary file 'GSE41605_merlin_final_orfs.bed.gz' includes the 751 ORFs of HCMV that were identified in this study.

ORGANISM(S): Human Herpesvirus 5

SUBMITTER: Noam Ginossar

PROVIDER: E-GEOD-41605 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Decoding human cytomegalovirus.

Stern-Ginossar Noam N Weisburd Ben B Michalski Annette A Le Vu Thuy Khanh VT Hein Marco Y MY Huang Sheng-Xiong SX Ma Ming M Shen Ben B Qian Shu-Bing SB Hengel Hartmut H Mann Matthias M Ingolia Nicholas T NT Weissman Jonathan S JS

Science (New York, N.Y.) 20121101 6110

The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like those of other complex viruses, our understanding of its protein coding potential is far from complete. We used ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified hundreds of previously unidentified open reading frames and confirmed a fraction by means of mass spectrometry. We found that regulated use of alternative t ...[more]

PMID: 23180859

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Dataset Information

Decoding human cytomegalovirus using ribosome profiling

Publications

Decoding human cytomegalovirus.

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