Transcription profiling of human CD43+ cells isolated from myelodysplastic syndrome patients and normal controls reveals involvement of interferon-stimulated genes and correlation to FAB subtype and karyotype.

ABSTRACT: 55 patients with MDS and 11 healthy controls were included in the study. At the time of investigation, 18 patients had RA, 19 RARS and 18 RAEB. The RAEB group was further subdivided into RAEB1 and RAEB2 for a selected part of the analysis. Bone marrow samples were obtained and CD34+ cells isolated from MDS patients and healthy controls using MACS magnetic cell separation columns. Total RNA was extracted using TRIZOL following the protocol supplied by the manufacturer. RNA was amplified using the Two-Cycle cDNA Synthesis and the Two-Cycle Target Labelling and Control Reagent packages (Affymetrix). Samples were hybridized to GeneChip Human Genome U133 Plus 2.0 arrays. Affymetrix CEL files were pre-processed using Robust MultiChip Analysis (RMA).

ORGANISM(S): Homo sapiens

DISEASE(S): normal

SUBMITTER: Andrea Pellagatti 

PROVIDER: E-GEOD-4619 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress