Functional Characterization of Fission Yeast Transcription Factors by Overexpression Analysis

ABSTRACT: In Schizosaccharomyces pombe, over 90% of transcription factor genes are nonessential. Moreover, the majority do not exhibit significant growth defects under optimal conditions when deleted, complicating their functional characterization and target gene identification. Here, we systematically overexpressed 99 transcription factor genes with the nmt1 promoter. Screening the overexpression array revealed that 64 transcription factor genes exhibited reduced fitness when ectopically expressed. Cell cycle defects were also often observed. We further investigated three uncharacterized transcription factor genes (toe1+-toe3+) which displayed cell elongation when overexpressed. Ectopic expression of toe1+ resulted in a G1 delay while toe2+ and toe3+ overexpression produced an accumulation of sept

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Gordon Chua 

PROVIDER: E-GEOD-46811 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress