Transcriptome of phosphomimetic and phosphoablative kasB mutants of Mycobacterium tuberculosis

ABSTRACT: Mechanisms governing Mycobacterium tuberculosis acid-fastness and its capacity to induce long-term infections remain unknown. Serine/Threonine phosphorylation represents an emerging theme allowing mycobacteria to adapt their cell envelope structure/composition in response to environmental changes. We addressed whether phosphorylation of KasB, a mycolic acid biosynthetic enzyme, modulates M. tuberculosis pathogenicity. Phosphorylation of KasB occurred at Thr334 and Thr336 in vitro and in mycobacteria. A mutant strain bearing an kasB_T334D/T336D allele, mimicking constitutive KasB phosphorylation, was generated by specialized linkage transduction. This resulted in shortened mycolic acids and the lack of trans-cyclopropanation. Structural/modeling analyses revealed Thr334 and Thr336 in the vi

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Brian Weinrick 

PROVIDER: E-GEOD-47640 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress