Mammary development during pregnancy (controlateral control vs. cleared fat pad) to define transdifferentiation factors

ABSTRACT: Tissue from cleared mammary fat pad and sham-operated controlateral control was removed before and at 10, 15,17, and 19 days of pregnancy. Cleared vs. controlateral tissue was hybridized in dye-swap design to in-house cDNA microarry platform. This design aims to identify factors signaling from the epithelial part of the mammary gland to the surrounding fat pad to initiate adipo-epithelial transdifferentiation. We surgically removed the epithelial part of the 4th mammary gland (including the nipple and the rudimentary ductal tree) in three weeks old CD1 mice where the ductal anlage is confined to the proximal part of the mammary fat pad close to the nipple. The controlateral gland was sham-operated as control. This leaves a cleared mammary fat pad in its endogenous environment. The dissecte

ORGANISM(S): Mus musculus

SUBMITTER: Andreas Prokesch 

PROVIDER: E-GEOD-50447 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress