Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Brain tumor initiating cell response to microglia

ABSTRACT: Determination of the mechanism by which microglia regulate growth of brain tumor initiating cells (BTICs) and differentiation. Results identify the factors involved in the regulation and provide mechanistic basis. We subjected brain tumor initiating cells to microarray to determine the genes involved in BTICs growth and differentiation when exposed to microglia conditioned medium (MCM) for 6h. BTICs were grown in MCM or control medium for 6h, and media was removed and then the cells were subjected for RNA extraction and hybridization on Affymatrix microarrays. Three (3) control and four (4) MCM samples were generated. The overall objective was to collect the RNA from the MCM exposed differentiated BTICs.

ORGANISM(S): Homo sapiens

SUBMITTER: Voon Yong

PROVIDER: E-GEOD-52127 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Therapeutic activation of macrophages and microglia to suppress brain tumor-initiating cells.

Sarkar Susobhan S Döring Axinia A Zemp Franz J FJ Silva Claudia C Lun Xueqing X Wang Xiuling X Kelly John J Hader Walter W Hamilton Mark M Mercier Philippe P Dunn Jeff F JF Kinniburgh Dave D van Rooijen Nico N Robbins Stephen S Forsyth Peter P Cairncross Gregory G Weiss Samuel S Yong V Wee VW

Nature neuroscience 20131208 1

Brain tumor initiating cells (BTICs) contribute to the genesis and recurrence of gliomas. We examined whether the microglia and macrophages that are abundant in gliomas alter BTIC growth. We found that microglia derived from non-glioma human subjects markedly mitigated the sphere-forming capacity of glioma patient-derived BTICs in culture by inducing the expression of genes that control cell cycle arrest and differentiation. This sphere-reducing effect was mimicked by macrophages, but not by neu ...[more]

PMID: 24316889

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Dataset Information

Brain tumor initiating cell response to microglia

Publications

Therapeutic activation of macrophages and microglia to suppress brain tumor-initiating cells.

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