Adaptation of Bacillus subtilis carbon core metabolism to simultaneous nutrient limitation and osmotic challenge

ABSTRACT: Here, we investigated for the first time the systems-wide response of B. subtilis to different simultaneous stresses, i.e. nutrient limitation and high osmolarity. To address the anticipated complexity of the cellular response networks, we combined chemostat experiments under conditions of carbon limitation, salt stress and osmoprotection with multi-omics analyses at the transcriptome, proteome, metabolome and fluxome levels. Our results indicate that the flux through central carbon and energy metabolism is very robust under all conditions studied. The key to achieve this robustness is the adjustment of the biocatalytic machinery to compensate for solvent-induced impairment of enzymatic activities during osmotic stress. The accumulation of the exogenously provided osmoprotectant glycine be

ORGANISM(S): Bacillus subtilis

SUBMITTER: Ulrike MM-CM-$der 

PROVIDER: E-GEOD-53333 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress