Genomewide demarcation of RNA PolII transcription units by physical fractionation of chromatin- ALL Arrays

ABSTRACT: Epigenetic modifications of chromatin serve an important role in regulating the expression and accessibility of genomic DNA. We report here a genomewide approach for fractionating yeast chromatin into two functionally distinct parts, one containing RNA polymerase II transcribed sequences, and the other comprising noncoding sequences and genes transcribed by RNA polymerases I and III. Noncoding regions could be further fractionated into promoters and segments lacking promoters. The observed separations were apparently based on differential crosslinking efficiency of chromatin in different genomic regions. The results reveal a genomewide molecular mechanism for marking promoters and genomic regions that have a license to be transcribed by RNA polymerase II, a previously unrecognized level of

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jason Lieb 

PROVIDER: E-GEOD-5662 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress