Next Generation Sequencing Facilitates Quantitative Analysis of Vitis flower Wild Type and the domesticated Vitis Transcriptomes

ABSTRACT: Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived flower development transcriptome profiling (RNA-seq) of two subspecies Methods: Flower mRNA profiles of wild-type (WT) four developmental stages and the same stages of Vitis vinifera subp vinifera were generated by deep sequencing using Illumina. Initial quality assessment was based on data passing the Illumina Chastity filtering. Subsequently, reads containing adapters and/or PhiX control signal were removed using an in-house filtering protocol. The second quality assessment was based on the remaining reads using the FASTQC quality control tool version 0.10.0. qRT–PCR validation was performed using EvaGreen assays. Results: Using an

ORGANISM(S): Vitis vinifera

SUBMITTER: Margarida Rocheta 

PROVIDER: E-GEOD-56844 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress