Transcription profiling of mouse fibroblasts to examine the cellular transformation induced by Rho subfamily GTPases

ABSTRACT: We have used microarray technology to identify the transcriptional targets of Rho subfamily GTPases. This analysis indicated that murine fibroblasts transformed by these proteins show similar transcriptomal profiles. Functional annotation of the regulated genes indicate that Rho subfamily GTPases target a wide spectrum of biological functions, although loci encoding proteins linked to proliferation and DNA synthesis/transcription are up-regulated preferentially. Rho proteins promote four main networks of interacting proteins nucleated around E2F, c-Jun, c-Myc, and p53. Of those, E2F, c-Jun and c-Myc are essential for the maintenance of cell transformation. Inhibition of Rock, one of the main Rho GTPase targets, leads to small changes in the transcriptome of Rho-transformed cells. Roc

ORGANISM(S): Mus musculus

SUBMITTER: Xose Garcia Bustelo 

PROVIDER: E-GEOD-5913 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress