Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from primary rat aortic smooth muscle cells

ABSTRACT: Electrochemical gradients of monovalent cations across the plasma membrane (high intracellular potassium, [K+]i vs low intracellular sodium, [Na+]i) are created by the Na+,K+-pump and determine a large variety of physiologically important processes. We hypothesized that transcriptomics changes triggered by hypoxia are at least partially caused by Na+i/K+i-mediated excitation-transcription coupling . We used microarrays for identification and functional characterization of [Na+]i/]K+]i-sensitive transcriptome in RASMC Quiescent cells were washed with K+-free DMEM and incubated for 6 hr at 37oC in a humidified atmosphere with 5% CO2/balance air in control DMEM, K+-free DMEM and DMEM containing 3 mM ouabain. Total RNA was extracted from cells grown in 6-well plates with TRIzolM-BM-. reagent and purified with RNeasyM-BM-. MinElute cleanup kit, following the manufacturersM-bM-^@M-^Y protocols. Microarray experiments were performed with GeneChipM-BM-. Rat Gene 1.0 ST array.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Sergei Orlov

PROVIDER: E-GEOD-61131 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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