High resolution specificity of DNA sequencing highlights alternative mode of lac repressor binding

ABSTRACT: In this work we introduce a new high-throughput method, Spec-seq, that directly measures specificity by sequencing. It has several advantages over existing methods to quantify large collections (thousands) of binding site energies in one experiment. Using lac repressor as an example, we show that this method has excellent reproducibility giving energy measurements generally consistent within about 0.1kT. While not measuring in parallel as many different sequences as some of the higher throughput methods, we obtain high accuracies because we measure exactly what is necessary, the relative affinity to a large collection of sequences, without any mathematical fitting procedures or approximations required. It is similar to MITOMI in the number of different sites that can be analyzed in paralle

ORGANISM(S): Escherichia coli K-12

SUBMITTER: Gary Stormo 

PROVIDER: E-GEOD-61223 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress