Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Effect of Drosophila immune proteins on gene expression in E. coli

ABSTRACT: Interaction between the host and invading pathogen determines the fate of both organisms during the infectious state. The host is equipped with a battery of immune reactions, while the pathogen displays a variety of mechanisms to compromise host immunity. Although bacteria alter their pattern of gene expression when they enter host organisms, studies to elucidate the mechanism behind this are only in their infancy. In the present study, we examined the possibility that host immune proteins directly participate in the change of gene expression in bacteria. To this end, Escherichia coli was treated with a mixture of the extracellular region of membrane-bound peptidoglycan recognition protein LC (PGRP-LC) and the antimicrobial peptide attacin of Drosophila, and subsequently subjected to DNA microarray analysis for the repertoire of mRNA. We identified nearly 200 genes whose mRNA increased after the treatment, and at least four of them were induced in response to PGRP-LC. One such gene, lipoprotein-encoding nlpI, showed a transient increase of its mRNA level in adult flies depending on PGRP-LC, and NlpI-lacking E. coli had a smaller pathogenic effect with lowered growth/viability than the parental strain in adult flies. These results suggest that a host immune receptor triggers a change of gene expression in bacteria simultaneously to their recognition of the invader and induction of immune responses. The E. coli strain BW25113 (2 x 10^9) suspended with insect saline (0.13 M NaCl, 4.7 mM KCl, 1.9 mM CaCl2), was incubated with a mixture of GST-attacin (0.125 Î¼M), GST-PGRP-LCa (0.5 Î¼M), GST-PGRP-LCx (1 Î¼M), and GST-PGRP-LCy (0.5 Î¼M) for 10 min at room temperature. As a negative control, incubation of E. coli was done in the presence of GST alone (3 Î¼M).

ORGANISM(S): Escherichia coli

SUBMITTER: Yuji Nakai

PROVIDER: E-GEOD-61604 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Peptidoglycan recognition protein-triggered induction of Escherichia coli gene in Drosophila melanogaster.

Kong Qingquan Q Nakai Yuji Y Kuroda Nanae N Shiratsuchi Akiko A Nagaosa Kaz K Nakanishi Yoshinobu Y

Journal of biochemistry 20150109 6

Interaction between the host and pathogen determines the fate of both organisms during the infectious state. The host is equipped with a battery of immune reactions, while the pathogen displays a variety of mechanisms to compromise host immunity. Although bacteria alter their pattern of gene expression in host organisms, studies to elucidate the mechanism behind this are only in their infancy. We here examined the possibility that host immune proteins directly participate in the change of gene e ...[more]

PMID: 25577644

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Dataset Information

Effect of Drosophila immune proteins on gene expression in E. coli

Publications

Peptidoglycan recognition protein-triggered induction of Escherichia coli gene in Drosophila melanogaster.

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