Zelda overcomes the high intrinsic nucleosome barrier at enhancers during Drosophila zygotic genome activation

ABSTRACT: A central question in biology is how enhancers are made accessible. The Drosophila embryo is a good model system to study this question as the gene regulatory networks regulating early developmental events have been well characterized. Zelda (Zld) is a uniformly distributed transcription factor (TF) integral to these networks, acting prior to and in collaboration with the patterning TFs to regulate target enhancers. Here we test the hypothesis that Zld directs TF binding, examplified by Dorsal (Dl) which patterns the dorsoventral axis, across the genome by displacing nucleosomes at enhancers. By performing ChIP-seq and MNase-seq experiments on early embryos with or without Zld, we demonstrated that early enhancers are characterized by an intrinsically high nucleosome barrier, which is ove

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Christine Rushlow 

PROVIDER: E-GEOD-65441 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress