Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Expression data from MSC-treated monocytes

ABSTRACT: Chronic inflammation leading to pro-inflammatory macrophage infiltration contributes to the pathogenesis of type 2 diabetes and subsequently the development of diabetic nephropathy. Mesenchymal stem cells (MSCs) possess unique immunomodulatory and cytoprotective properties making them an ideal candidate for therapeutic intervention We used microarrays to detail changes in the gene expression profile of monocytes isolated from type 2 diabetic patients with end-stage renal disease and non-diabetic control subjects following co-culture with MSCs. Control blood samples were obtained from 4 donors from the Australian Red Cross Blood Service. Blood was also obtained from 5 diabetic patients with end-stage renal disease receiving haemodialysis at the Monash Medial Centre. CD14+ monocytes were isolated from blood using microbeads and co-cultured for 48 hours with and without human bone marrow-derived MSCs using an in vitro transwell system. An Affymetrix GeneChip Human Gene 2.0 ST array was used.

ORGANISM(S): Homo sapiens

SUBMITTER: Tyrone Chen 

PROVIDER: E-GEOD-65561 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Human mesenchymal stem cells alter the gene profile of monocytes from patients with Type 2 diabetes and end-stage renal disease.

Wise Andrea F AF   Williams Timothy M TM   Rudd Stephen S   Wells Christine A CA   Kerr Peter G PG   Ricardo Sharon D SD  

Regenerative medicine 20151106 2

<h4>Aim</h4>Macrophage infiltration contributes to the pathogenesis of Type 2 diabetes. Mesenchymal stem cells (MSCs) possess immunomodulatory properties, making them an ideal candidate for therapeutic intervention. This study investigated whether MSCs can modulate the phenotype of monocytes isolated from Type 2 diabetic patients with end-stage renal disease.<h4>Materials & methods</h4>Monocytes from control (n = 4) and Type 2 diabetic patients with end-stage renal disease (n = 5) were assessed  ...[more]

Similar Datasets

Unknown Expression data from MSC-treated monocytes
2015-11-10 | GSE65561 | GEO
Transcriptomics MiRNA arrays on human bone marrow-derived mesenchymal stem/stromal cells
2020-07-28 | E-MTAB-9418 | biostudies-arrayexpress
Transcriptomics Expression data from control and ZNF145-overexpressing human mesenchymal stem cells (MSCs)
2010-12-22 | E-GEOD-26272 | biostudies-arrayexpress
Transcriptomics Effects of dynamic compressive loading on MSC chondrogenesis
2010-11-01 | E-GEOD-18879 | biostudies-arrayexpress
Transcriptomics Molecular characterization of immortalization of human MSCs by a combination of p53 knockdown and TERT overexpression
2011-04-13 | E-GEOD-28546 | biostudies-arrayexpress
Transcriptomics EID1 knochdown by small RNA induces the large-scale conversion of adipose-derived mesenchymal stem cells into hematopoietic stem cells
2012-12-01 | E-GEOD-42650 | biostudies-arrayexpress
Transcriptomics miR-146a-5p circuitry uncouples cell proliferation and migration, but not differentiation, in human mesenchymal stem cells
2013-09-05 | E-GEOD-46989 | biostudies-arrayexpress
Transcriptomics Mesenchymal Stem Cells (MSCs): Control vs. PTH-stimulated
2014-02-01 | E-GEOD-43148 | biostudies-arrayexpress
Transcriptomics Gene expression compare between liver cancer associated mesenchymal stem cells (LC8-MSC) and liver normal mesenchymal stem cells (LN8-MSC)
2012-11-17 | E-GEOD-42357 | biostudies-arrayexpress
Transcriptomics scRNA-seq of Multi-lineage differentiating Stress Enduring (Muse) cell and non-Muse cells isolated from bone marrow-derived mesenchymal stem cells
2022-10-26 | E-MTAB-10854 | biostudies-arrayexpress