Distinct mechanisms of inadequate erythropoiesis induced by tumor necrosis factor alpha or malarial pigment

ABSTRACT: The role of infection in erythropoietic dysfunction is poorly understood. In children with P. falciparum malaria, the by-product of hemoglobin digestion in infected red cells (hemozoin) is associated with the severity of anemia which is independent of circulating levels of the inflammatory cytokine tumor necrosis alpha (TNF-alpha). To gain insight into the common and specific effects of TNF-alpha and hemozoin on erythropoiesis, we studied the gene expression profile of purified primary erythroid cultures exposed to either TNF-alpha (10ng/ml) or to hemozoin (12.5microgram/ml heme units) for 24 hours. Perturbed gene function was assessed using co-annotation of associated gene ontologies and expression of selected genes representative of the profile observed was confirmed by real time PCR (rt

ORGANISM(S): Homo sapiens

SUBMITTER: Alison Merryweather-Clarke 

PROVIDER: E-GEOD-65577 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress