The NuA4 complex promotes Translesion Synthesis (TLS) - mediated DNA damage tolerance

ABSTRACT: DNA lesions can block a replication fork, leading to its collapse and gross chromosomal rearrangements. To circumvent such outcomes, DNA damage tolerance (DDT) pathways become engaged, allowing the replisome to bypass the lesion and complete S phase in the presence of unrepaired damage. Here we demonstrate a newly identified role for NuA4, including complex components Esa1 and Yng2, on the Translesion Synthesis (TLS) branch of DDT. Moreover, Our data suggest that NuA4 functionality within the tolerance pathway is likely direct as genome-wide transcriptional analysis with esa1-L254P mutants showed little changes in the expression of TLS factors compared to wild type during MMS treatment. When Yng2 expression is restricted to G2/M, cell viability and mutagenesis rates are restored to the lev

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jennifer Cobb 

PROVIDER: E-GEOD-66176 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress