Transcription profiling of human naive T cells isolated from peripheral blood treated with 1,25 (OH)2 vitamin D3 induces expression of CCR10 and other genes

ABSTRACT: Human naive T cells from peripheral blood were cultured in 24 wells coated with anti-CD3 and anti-CD28 antibodies in the presence or absence of retinoid acid, IL-12, and 1,25 (OH)2 vitamin D3. The T cells were FACS-sorted based on expression of CD3, integrin alpha4beta7, cutaneous lymphocyte antigen (CLA) and chemokine receptor 10. This serie includes microarray data from stimulated T cells under indicated conditions. Experiment Overall Design: Total RNA was isolated from these sorted T cells using Invitrogen RNeasy mini-kit. The quality of RNA was prechecked by Agilent BioAnalyzer. Double-stranded cDNA was synthesized, and transcribed into Biotin-labeled cRNA, which was fragmented before hybridization. All the procedure was performed in the Stanford University PAN facility.

ORGANISM(S): Homo sapiens

SUBMITTER: Junliang Pan 

PROVIDER: E-GEOD-6743 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress