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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from foam cells of apolipoprotein E-deficient mice

ABSTRACT: Hypercholesterolemai is a major contributor to atherosclerosis development. To assess the effects of hypercholesterolemia on the transcriptional profiling in foam cells, mice were fed regular chow, or WD for 2 or 14 weeks prior to sacrifice. We used microarrays for broad analysis of gene expression in the 3 experimental groups. Each group included 5 mice. ApoE-/- mice were fed regular mouse chow to the age of 22 weeks (CHOW) or a western-type diet for 2 weeks between weeks 22 and 24 (2w-WD) or for 14 weeks between weeks 8 and 22 (14w-WD). Foam cells in cross-sections of the aourtic sinus were isolated by laser-capsure microdissection (n=5 per group). RNA was amplified by in vitro transcription, biotinylated, and hybridized to Affymetrix Mouse Genome 430 2.0 Arrays.

ORGANISM(S): Mus musculus

SUBMITTER: Antonio Paul

PROVIDER: E-GEOD-70619 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from foam cells of apolipoprotein E-deficient mice

Project description:Hypercholesterolemai is a major contributor to atherosclerosis development. To assess the effects of hypercholesterolemia on the transcriptional profiling in foam cells, mice were fed regular chow, or WD for 2 or 14 weeks prior to sacrifice. We used microarrays for broad analysis of gene expression in the 3 experimental groups. Each group included 5 mice.

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