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Transcription profiling of mouse heart after dietary supplementation of vitamin E in the mouse heart to investigate age-related transcriptional changes

ABSTRACT: To investigate the global effects of vitamin E supplementation on heart aging, we used high-density oligonucleotide arrays to measure transcriptional alterations in 30-month-old B6C3F1 mice supplemented with Î±- and Î³-tocopherol since middle age (15 months). Experiment Overall Design: Gene expression profiles were obtained from 5-month-old controls, 30-month-old controls and 30-month-old mice supplemented with Î±-tocopherol (1g/kg), or a mixture of Î±- and Î³-tocopherol (500mg/kg of each tocopherol).

ORGANISM(S): Mus musculus

SUBMITTER: Sang-Kyu Park

PROVIDER: E-GEOD-8146 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of mouse heart and brain to investigate age-related transcriptional changes and the effect of dietary supplementation of vitamin E

Project description:This SuperSeries is composed of the following subset Series:; GSE8146: Age-related transcriptional changes and the effect of dietary supplementation of vitamin E in the mouse heart; GSE8150: Age-related transcriptional changes and the effect of dietary supplementation of vitamin E in the mouse brain Experiment Overall Design: Refer to individual Series

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Transcription profiling of mouse brain to identify age-related transcriptional changes and the effect of dietary supplementation of vitamin E

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Transcription profiling of chicken liver after alpha- and gamma-tocopherol supplementation in case of increased oxidative stress susceptibility

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2017-02-28 | E-MTAB-4507 | biostudies-arrayexpress

Enhanced Phospholipase A2 group 3 expression by oxidative stress decreases the insulin-degrading enzyme

Project description:Oxidative stress has a ubiquitous role in neurodegenerative diseases and oxidative damage in specific regions of the brain is associated with selective neurodegeneration. We previously reported that Alzheimer disease (AD) model mice showed decreased insulin-degrading enzyme (IDE) levels in the cerebrum and accelerated phenotypic features of AD when crossbred with alpha-tocopherol transfer protein knockout (Ttpa-/-) mice. To further investigate the role of chronic oxidative stress in AD pathophysiology, we performed DNA microarray analysis using young and aged wild-type mice and aged Ttpa-/- mice. Among the genes whose expression changed dramatically was Phospholipase A2 group 3 (Pla2g3); Pla2g3 was identified because of its expression profile of cerebral specific up-regulation by chronic oxidative stress in silico and in aged Ttpa-/- mice. Immunohistochemical studies also demonstrated that human astrocytic Pla2g3 expression was significantly increased in human AD brains compared with control brains. Moreover, transfection of HEK293 cells with human Pla2g3 decreased endogenous IDE expression in a dose-dependent manner. Our findings show a key role of Pla2g3 on the reduction of IDE, and suggest that cerebrum specific increase of Pla2g3 is involved in the initiation and/or progression of AD. Gene expression in cerebral cortex and cerebellum of mice were determined using Agilent chips. To ensure higher quality results in gene expression data, we conducted microarrays on 4 mice per group. Young mice were 2 months old and the other aged mice were 29 months old at the time of use. Data were standardized using global normalization and pro-cessed by R-program. An absolute fold change threshold of greater than 1.5 was required to be considered for further analyses. Expression values were in log2 scale.

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2010-12-03 | E-GEOD-25767 | biostudies-arrayexpress

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