Transcription profiling of mouse T-cell thymocytes and bone marrow-derived macrophages dervied from Upf2fl/fl;LysMCre and Upf2fl/fl;LckCre mice to test the importance of nonsense-mediated mRNA decay on global transcription

ABSTRACT: Knockdown experiments in transformed human cells, suggested that nonsense-mediated mRNA decay (NMD) components had extensive effects on the regulation of a significant fraction of the transcriptome In order to test the importance of NMD on global transcription we subjected BMDMs and T-cell thymocytes derived from Upf2fl/fl;LysMCre and Upf2fl/fl;LckCre mice, respectively, to microarray-based gene expression profiling. Both these cell types display strong, if not complete, reduction of NMD as assayed by transfection with reporter constructs or sequencing of Tcrb transcripts. Upf2¯/¯ BMDMs displayed significant changes in the expression of 182 genes (234 probe sets) of which 179 were upregulated.

ORGANISM(S): Mus musculus

SUBMITTER: Charlotte Schjerling 

PROVIDER: E-MEXP-1371 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress