Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Heterogeneity in the hepatic stellate cell population in mouse liver

ABSTRACT: We utilized two methods of hepatic stellate cell (HSC) isolation, which leverage distinct properties of these cells, including their vitamin A content and collagen expression. HSCs were isolated either from wild type (WT) mice in the C57BL/6 genetic background by flotation in a Nycodenz density gradient, followed by fluorescence activated cell sorting (FACS) based on vitamin A autofluorescence, or from collagen-green fluorescent protein (GFP) mice by FACS based on GFP expression, from a GFP transgene driven by the collagen I promoter. Array analysis was conducted on the Affymetrix Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA).

ORGANISM(S): Mus musculus

SUBMITTER: Diana D'Ambrosio

PROVIDER: E-MEXP-3231 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Distinct populations of hepatic stellate cells in the mouse liver have different capacities for retinoid and lipid storage.

D'Ambrosio Diana N DN Walewski José L JL Clugston Robin D RD Berk Paul D PD Rippe Richard A RA Blaner William S WS

PloS one 20110916 9

<h4>Unlabelled</h4>Hepatic stellate cell (HSC) lipid droplets are specialized organelles for the storage of retinoid, accounting for 50-60% of all retinoid present in the body. When HSCs activate, retinyl ester levels progressively decrease and the lipid droplets are lost. The objective of this study was to determine if the HSC population in a healthy, uninjured liver demonstrates heterogeneity in its capacity for retinoid and lipid storage in lipid droplets. To this end, we utilized two methods ...[more]

PMID: 21949825

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Dataset Information

Heterogeneity in the hepatic stellate cell population in mouse liver

Publications

Distinct populations of hepatic stellate cells in the mouse liver have different capacities for retinoid and lipid storage.

Similar Datasets

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