RNAi knock down of export factors in Drosphila Schneider cell lines

ABSTRACT: Six different endogenous Drosophila proteins (all potential export factors) have been inactivated using RNAi and leptomycin B (LMB) treatments. Five different endogenous Drosophila proteins (all potential export factors) have been targeted by RNAi. The effect on mRNA export is analyzed by monitoring the levels of mRNAs in cytoplasmic and total samples. Samples from knock-downs are compared to samples obtained from (GFP transfected) control cells processed in parallel. [The two samples compared have the same name, but the suffix _KD for knock down and _ctrl for the control]. The potential export factors targeted by RNAi are: NXF1, NXF2, NXF3, p15 and UAP56. In an additional control experiment the translation initiation factor eIF4G was targeted by RNAi (to control for the specificity of the

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Luis Teixeira 

PROVIDER: E-MEXP-4 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress