RNA-Seq analysis of Bacillus methanolicus MGA3 grown with and without 5-aminovalerate to investigate the transcriptional response of Bacillus methanolicus to 5-aminovalerate.

ABSTRACT: Bacillus methanolicus MGA3, which has a low tolerance for 5-aminovalerate. To investigate the transcriptional response of Bacillus methanolicus to 5-aminovalerate transcriptomic analysis by differential RNA-Seq in the presence and absence of 5AVA was perfromed. In detail: B. methanolicus cultures were grown in MVcM or MVcMY media containing 200 mM methanol supplemented with and without 50 mM 5AVA, respectively. Cells were harvested in the mid log phase at an OD600 of 0.6 and isolation of total RNA isolation was performed individually for each cultivation condition. Isolated RNA samples from B. methanolicus MGA3 were used in biological triplicates for the cDNA library preparation prior to sequencing. Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove

INSTRUMENT(S): Illumina HiSeq 1500

ORGANISM(S): Bacillus methanolicus MGA3

SUBMITTER: Carsten Haupka 

PROVIDER: E-MTAB-10101 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress