Microarray of MDV infected cultured chicken bursa cells

ABSTRACT: B lymphocytes from the chicken Bursa of Fabricius were isolated and cultured in the presence of CD40L. Cells were infected with a GFP fluorescent reporter virus of the very virulent Marek's disease virus (vvMDV) strain RB-1B (RB-1B_UL47-GFP, see doi: 10.1073/pnas.1424420112). After 24h viable infected (GFPpositive) and uninfected (GFPnegative) B-cells were sort purified from these cultures. Viable B cells, purified from uninfected cultures served as control. 3x10^6 cells per sample were subjected to RNA isolation and microarray analysis. Goal of the experiment was to elucidate the reaction of chicken B cells, primary target cells of MDV upon infection with this virus.

INSTRUMENT(S): Bioanalyzer „Agilent 2100 Expert, Agilent G205C Scanner, 3µm

ORGANISM(S): Gallus gallus

SUBMITTER: Sonja Härtle 

PROVIDER: E-MTAB-10702 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress