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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Three distinct patterns of histone H3Y41 phosphorylation mark active genes

ABSTRACT: The aim of the project was to identify the global distribution of H3Y41ph and correlate its presence with the transcription factor STAT5 and the active histone modification H3K4me3. This was done in human erythroid leukaemia cells (HEL). HEL cells were treated with DMSO or a JAK2 inhibitor (TG101209) for 4 hours they were then crosslinked with formaldehyde and Chromatin immunoprecipitation was performed using antibodies against the proteins of interest. The DNA was then sequenced on the solexa platform. The analysis includes 7 samples: (1) H3Y41ph with DMSO, (2) H3Y41ph with TG101209, (3) STAT5 with DMSO, (4) STAT5 with TG101209, (5) IgG with DMSO, (6) H3K4me3 with DMSO, (7) independent replicate with H3Y41ph in DMSO

ORGANISM(S): Homo sapiens

SUBMITTER: Mark Dawson

PROVIDER: E-MTAB-1096 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Three distinct patterns of histone H3Y41 phosphorylation mark active genes.

Dawson Mark A MA Foster Samuel D SD Bannister Andrew J AJ Robson Samuel C SC Hannah Rebecca R Wang Xiaonan X Xhemalce Blerta B Wood Andrew D AD Green Anthony R AR Göttgens Berthold B Kouzarides Tony T

Cell reports 20120920 3

The JAK2 tyrosine kinase is a critical mediator of cytokine-induced signaling. It plays a role in the nucleus, where it regulates transcription by phosphorylating histone H3 at tyrosine 41 (H3Y41ph). We used chromatin immunoprecipitation coupled to massively parallel DNA sequencing (ChIP-seq) to define the genome-wide pattern of H3Y41ph in human erythroid leukemia cells. Our results indicate that H3Y41ph is located at three distinct sites: (1) at a subset of active promoters, where it overlaps w ...[more]

PMID: 22999934

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