Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

9 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Feedback from dFOXO in the gut and fat body

ABSTRACT: Whole fly transcript profiles of changes upon induction of dFOXO in the gut and fat body using S1106>dFOXO flies and how this response changes when the feedback to foxo is removed in the foxo null background

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Nazif Alic

PROVIDER: E-MTAB-1232 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling by array of adult Drosophila melanogaster gut and fat body in S1106>dfoxo females after 5 days of induction of transgene expression by RU486 feeding.

Project description:Transcription profiling by array of adult abdominal/thorasic fat body or midgut in S1106>dfoxo females after 5 days of induction of transgene expression by RU486 feeding.

2014-08-18 | E-MTAB-1020 | biostudies-arrayexpress

ChIP-chip by array of GFP-tagged dFOXO expressed in Drosophila melanogaster adult gut and fat body

Project description:ChIP-chip by array of GFP-tagged dFOXO expressed in Drosophila melanogaster adult gut and fat body

2014-08-18 | E-MTAB-1021 | biostudies-arrayexpress

dFOXO targets in Drosophila melanogaster

Project description:dFOXO targets in adult Drosophila melanogaster females, and the effect of insulin signalling and stress on binding. The experimets determined the binding locations of dFOXO in the whole adult female fly using ChIP-chip. The protocol was validated using mock conditions: pre-immune serum or IP on chromatin from foxo null flies. The response of this binding to stress induced by treatment of flies with paraquat or by their exposure to starvation, as well as the response to an insulin-signalling-reducing genetic manipulation (over-expression of dominant negative form of the insulin receptor), was determined.

2011-06-18 | E-TABM-751 | biostudies-arrayexpress

Transcriptional analysis of dInR and dfoxo epistasis in Drosophila melanogaster

Project description:Transcriptional analysis of dInR and dfoxo epistasis in Drosophila melanogaster. The experiment was performed to examine which parts of the transcriptional response to a reduction in insulin signalling in an adult female fly depend on the presence of the dfoxo transcription factor. Whole fly transcriptome was determined with flies over-expressing a dominant negative form of the insulin receptor, or the wild-type fly, in presence or absence of dfoxo.

2011-06-18 | E-TABM-757 | biostudies-arrayexpress

Induction of AopACT in adult Drosophila abdominal fat body

Project description:Transcript profiling of adult abdominal fat body in S1106>AopACT females after 5 days of induction of transgene expression by RU486 feeding.

2014-08-18 | E-MTAB-927 | biostudies-arrayexpress

AopACT binding in adult gut and fat body upon its induction in Drosophila melanogaster

Project description:ChIP-chip on FLAG-tagged AopACT expressed in adult gut and fat body.

2014-08-18 | E-MTAB-1306 | biostudies-arrayexpress

Titered FOXO overexpression maintains cardiac proteostasis and ameliorates age-associated functional decline

Project description:Heart performance declines with age. Reduced protein quality control (PQC) due to decreased function of the ubiquitin/proteasome system (UPS), autophagy, and/or chaperone-mediated protein refolding is a likely contributor to age-associated cardiac performance decline. The transcription factor FOXO participates in the regulation of genes involved PQC and a host of other processes. Here, the effect of cardiac-restricted dFOXO overexpression was investigated in Drosophila, a genetically pliable and rapidly aging model. Modest dFOXO overexpression in the heart was protective, ameliorating functional decline with age. Increased expression of genes associated predominantly with UPS relative to other PQC components accompanied dFOXO-mediated cardioprotection, which was corroborated by a significant decrease in ubiquitinated myocardial proteins. In agreement, knockdown of upregulated UPS components seemingly induced premature aging. Despite these findings, excessive dFOXO overexpression or knockdown proved detrimental to heart function and overall organismal development. This study highlights Drosophila as a model of cardiac aging and FOXO as a tightly-regulated mediator of proteostasis and heart performance over time. Two replicates of 4 different samples were analyzed. Two of these samples were controls (GMH5 x yw 1 week and GMH5 x yw 5 week).

2016-07-01 | E-GEOD-73205 | biostudies-arrayexpress

Genome-wide analysis of dFOXO binding sites in Drosophila

Project description:To understand how reduced insulin/IGF-1 signaling extends Drosophila lifespan through its downstream transcription factor dFOXO. We conducted ChIP analysis with a dFOXO antibody followed by Illumina high-throughput sequencing from chico heterozygous mutants, which are long-lived and normal sized, and from adult flies with ablated insulin producing cells (IPCs), which are also long-lived. dFOXO bound at promoters of 273 genes common among these genotypes, thus potentially enriching for shared factors in control of aging.

2013-09-20 | GSE44686 | GEO

Effect of aging and dFOXO knockout on small RNAs

Project description:To examine the effect of loss of dFOXO on the small RNA landscape of aging animals, we sequenced total small RNA collected from whole wildtype (wDah) and dFOXO-null (dfoxoΔ94) flies at young (5-6 days) and old (31 days) age.

2022-09-04 | GSE212493 | GEO

Effect of aging and dFOXO knockout on small RNA in Ago1 and Ago2 RNA-induced silencing complexes

Project description:To examine the effect of loss of dFOXO on Ago1 and Ago2 RISC loading in aging animals, we sequenced RNA immunoprecipitated from Ago1 and Ago2 RISC collected from whole wildtype (wDah) and dFOXO-null (dfoxoΔ94) flies at young (5 days) and old (35 days) age. dFOXO is a transcription factor that regulates Ago1 and Ago2 as well as lifespan.

2022-09-04 | GSE212492 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data