DFOXO targets in Drosophila melanogaster

ABSTRACT: dFOXO targets in adult Drosophila melanogaster females, and the effect of insulin signalling and stress on binding. The experimets determined the binding locations of dFOXO in the whole adult female fly using ChIP-chip. The protocol was validated using mock conditions: pre-immune serum or IP on chromatin from foxo null flies. The response of this binding to stress induced by treatment of flies with paraquat or by their exposure to starvation, as well as the response to an insulin-signalling-reducing genetic manipulation (over-expression of dominant negative form of the insulin receptor), was determined.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Nazif Alic 

PROVIDER: E-TABM-751 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress