Functional microarray analysis of neonatal monocyte-derived macrophages differentiated in the presence or absence of human lactoferrin

ABSTRACT: Monocytes were isolated from umbilical cord blood of term born infants and differentiated with M-CSF in the presence or absence of human lactoferrin for 7 days. Afterwards cells were stimulated with LPS or left untreated, RNA isolated using a RNeasy Plus Micro Kit and samples submitted to microarray-analysis. The experiments were performed to investigate the functional impact of human lactoferrin, a transferrin glycoprotein highly prevalent in human breast-milk, on neonatal macrophages. The experimental model serves as a proxy for the proteins' immunoregulatory function within the developing neonatal gut.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Eigenschink 

PROVIDER: E-MTAB-13175 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress