Whole genome sequencing of unstable tebuconazole adaptors in Candida albicans
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ABSTRACT: Two tebuconazole adaptors, TJ1503 and TJ1669, were unstable. On YPD-agar plates, they gave rise to small (S) and large (L) colonies. We sequenced both types of colonies. TJ1503S and TJ1669S were the S colonies. TJ1503L and TJ1669L were the large colonies.
Project description:Candida albicans lab strain SC5314 was spotted on YPD plates supplemented with 0.25-16 μg/ml tebuconazole. Randomly at least 4 papillary colonies from each plate were sequenced.
Project description:H99 derived 2 ug/mL uniconazole adaptors: Approximately 1 million cells of Crypotoccus neoformans lab strain H99 were spread on YPD-agar plate supplemented with 2 ug/mL uniconazole. After 5 days incubation of the plate at 30℃, randomly 30 adaptors were chosen. 21 adaptors were resistant and were sequenced. H99 derived 0.25 ug/mL uniconazole adaptors: Cells of H99 were adjusted to 2500 cells/mL. The culture was supplemented with 0.25 ug/mL uniconazole. After 72 h incubation, the culture was washed, diluted and approximately 200 cells were spread on YPD-agar plate. Randomly 95 colonies were tested for resistance to uniconazole. One colony (TJ3832) was resistant and was sequenced.
Project description:Candida haemulonii wild type strain TJ180 were expsoured to 64 and 128 μg/ml fluconazole. 14 resistant mutants as well as the parent were sequenced.
Project description:From C. albicans reference strain SC5314, CDR1, CDR2, MDR1, CMP1, CNB1, CRZ1 were deleted. The deletion strains were spread on YPD plates supplemented with tebuconazole. Randomly 4 adaptors derived from each deletion strain were sequenced.
Project description:Gene AFR1 was deleted from Cryptococcus neoformans lab strain H99. Approximately 1 million cells of the deletion strain were spread on YPD-agar plate supplemented with 0.125ug/mL uniconazole. Randomly 30 adaptors were chosen. 16 adaptors were resistant to uniconazole and were sequenced.
Project description:Approximately 1 million cells of Crypotoccus neoformans lab strain H99 were spread on YPD-agar plate supplemented with 1 ug/mL uniconazole. After 5 days incubation of the plate at 30℃, randomly 30 adaptors were chosen and sequenced.
Project description:C. neoformans lab strain H99 was used to obtain uniconazole adaptors. Transcriptomes of 6 adaptors, each bearing a unique karyotype, were compared to the wild type H99.
Project description:YJB-T490 was spread on YPD-agar plates supplemented with miconazole (0.008-2ug/mL). Randomly 3 adaptors from each drug plate were sequenced. In addition, 2 miconazole adaptors derived from SC5314, and 6 adaptors derived from CDR1 deletion strain were also sequenced.
Project description:In Candida albicans, one strain (TJ1031) has segmental monosomy of chromosome R from rDNA to the right tolemere. This strain was unstable, yielding small and large colonies on YPD plate. Randomly 5 large colonies were sequenced.
Project description:In Candida albicans, aneuploids are unstable. When grown on YPD-agar plates, aneuploids exhibit colony size variations. In this study, 6 aneuploid strains were spread on YPD-agar plates. The large colonies from each strain were sequenced. The small colonies were sequenced in another project.