Microarray experiment of Candida albicans treated with synthetic peptides
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ABSTRACT: To investigate the mode of action of a novel class of antifungal synthtic acrylamide peptides, the pathogenic yeast, Candida albicans, was exposed to sublethal doses of peptides with different structures. The experiments include the most effective structure, LH, peptides with no antifungal effects (HEAm) and intermediates as well as controls.
Project description:To determine the potential mode of action of the new antifungal compound candidate keanumycin A, the C. albicans wild type SC5314 was exposed to a sublethal dose of the substance. Untreated samples serve as control.
Project description:Perforin is a major effector molecule of human natural killer (NK) cells. It can induce delayed growth of Candida albicans hyphae. Here, the fungal transcriptome was analyzed after a co-incubation with 500ng/µl Perforin.
Project description:Candida albicans were treated with a sublethal concentration of the antifungal Jagaricin for either a short time (30 min) or until an OD of 0.5 (indicating log growth) was reached. Controls were grown without any antifungal to determine cellular reactions to the compound.
Project description:Photosynthesis is affected by water deficiency (WD) stress, and nitric oxide (NO) is a free radical that participates in the photosynthesis process. Previous studies have suggested that NO regulates the excitation energy distribution of photosynthesis under WD stress. Here, quantitative phosphoproteomic profiling was conducted using isobaric tags for relative and absolute quantitation. Differentially phosphorylated protein species (DEPs) were identified in leaves of NO or polyethylene glycol (PEG)-treated wheat seedlings (D) and in control seedlings, 2,257 unique phosphorylated peptides and 2,416 phosphorylation sites were identified from 1,396 unique phosphoproteins. Of these, 96 DEPs displayed significant changes (≥ 1.50-fold, p < 0.01). These DEPs are involved in photosynthesis and signal transduction, etc. Furthermore, phosphorylation of several DEPs were up-regulated by both D and NO treatments, but down-regulated only in NO treatment. These differences affected the chlorophyll A-B binding protein, chloroplast post-illumination chlorophyll fluorescence increase protein, and SNT7, implying that NO indirectly regulated the absorption and transport of light energy in photosynthesis in response to WD stress. The significant difference of chlorophyll (Chl) content, Chl a fluorescence transient, photosynthesis index, and trapping and transport of light energy further indicated that exogenous NO under D stress enhanced the primary reaction of photosynthesis compared to D treatment. A putative pathway is proposed to elucidate NO regulation of the primary reaction of photosynthesis under WD.
Project description:The effect of the deletion of the hyphae-associated toxon of Candida albicans, camdidalysin, on yeast and hyphae tramscriptomes was tested with different mutants. Tested strains include the wild type (SC5314), deletion mutants of candidalyin and of ECE1 (the gene encoding the polyprotein that includes candidalysin) and an ECE1 revertant strain. Each were tested in yeast- and in hypae-supporting growth conditions.
Project description:The bacterium Lactobacillus rhamnosus antagonizes the fungus Candida albicans. The transcriptional response of C. albicans to the presence of L. rhamnosus in an in vitro infection model with and without antibiotic treatment was investigated using microarrays.
Project description:In this study, we quantified the relative protein levels from 12,627 genes across 32 normal human tissue types prepared by the GTEx project. Known and new tissue specific or enriched proteins were identified and compared to transcriptome data. Many ubiquitous transcripts are found to encode highly tissue specific proteins. Discordance in the sites of RNA expression and protein detection also revealed potential sites of synthesis and action of protein signaling molecules. Overall, these results provide an extraordinary resource, and demonstrate that understanding protein levels can provide insights into metabolism, regulation, secretome, and human diseases.
Project description:This work describes a previously unreported link between the chromatin states and protein abundance in cancer. We curated and studied a novel cohort of human thyroid tumors that allowed us to identify chromatin features that directly regulate protein expression. We believe this is the first study to establish chromatin states that reflect protein abundances in human cancer.
Project description:Arginine is associated with inflammation, cancer, and amino acid signaling, in part through the mTORC1 pathway. In cell-based assays and in the mouse, we found that arginine regulates nuclear levels of arginyl-tRNA synthetase (ArgRS), and that arginine depletion and inflammation reduced nuclear ArgRS in vivo. In the nucleus, ArgRS interacted and co-localized with the spliceosomal Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) that is crucial for the formation of nuclear speckle condensates. ArgRS mRNA silencing changed specific splice junction usages, and these inversely correlated with SRRM2-induced usage changes of the same junctions. The prominently affected genes encompassed components of the mTORC1 pathway and showed ArgRS, arginine, and SRRM2 are tied together as upstream regulators of nuclear condensate trafficking related to the physiological response to inflammatory injury. This study is the first to demonstrate a regulatory role for an aaRS in shaping nuclear condensate dynamics and RNA splicing.
Project description:Sak1 (orf19.3840) of Candida albicans was found to be a kinase which phosphorylates and thereby activates Snf1, a highly conserved regulator of nutrient stress responses. Accordingly, a sak1 deletion mutant failed to grow on many alternative (i.e., non-glucose) carbon sources, but also showed a filamentation defect upon growth under glucose limitation and exhibited reduced virulence. To better define the effects of Sak1 on C. albicans metabolic adaptations, these transcriptional analyses were performed in complex medium, using a sak1 deletion mutant in comparison to the wild type.