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Identification of MEX3D Substrate RNAs in the Testis using eCLIP-seq

ABSTRACT: Mouse testicular tissue was dissected and subjected to UV crosslinking, followed by extraction of protein-RNA complexes. MEX3D protein and its interacting RNAs were enriched using MEX3D antibody-conjugated beads. After PAGE electrophoresis, membrane transfer, and membrane excision, the target RNA was renatured and used for small RNA library construction and sequencing

INSTRUMENT(S): Illumina NovaSeq X

ORGANISM(S): Mus musculus

SUBMITTER: Zerui Wang

PROVIDER: E-MTAB-14968 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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