Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ChIPseq of RNA Exosome components in BMEL cell lines invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)

ABSTRACT: Chromatin association of several nuclear RNA exosome components and cofactors in BMEL cells is dependent on the presence of HP1 proteins. TKO2 cells invalidated for the expression of the three HP1 isoforms (Cbx1-/-; Cbx3-/-; Cbx5-/-) are compared to Het32 cells (Cbx1+/+; Cbx3+/+; Cbx5+/-) expressing HP1 as controls.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Christophe RACHEZ

PROVIDER: E-MTAB-15196 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

HP1, H3-K27me3, and H3-K9me3 Cut & Tag of Het (Cbx1+/+, Cbx3+/+, Cbx5+/-) and TKO (Cbx1-/-, Cbx3-/-, Cbx5-/-) bipotential mouse embryonic liver (BMEL) cells

Project description:Cut & Tag was used to localize the 3 mouse HP1 proteins (Cbx1,Cbx3,Cbx5) to the genome of BMEL (bipotential mouse embryonic liver) cells. Het BMEL cells are Cbx1+/+, Cbx3+/+, Cbx5+/- HP1-TKO (triple knock-out cells) are Cbx1-/-, Cbx3-/-, Cbx5-/-. The HP1_TKO cells were used to verify the specificity of the Cut & Tag signals. HP1 localization was compared with the chromatin localization of select RNA exosome subunits and adapters to support a role for HP1 in targeting the RNA exosome to specific chromatin regions.

2025-06-14 | E-MTAB-15189 | biostudies-arrayexpress

ATACseq in BMEL cells invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)

Project description:Chromatin accessibility assay by ATACseq in BMEL cells. Three clones (TKO1, TKO2, TKO3) of BMEL TKO cells invalidated for the expression of the three HP1 isoforms (Cbx1-/-; Cbx3-/-; Cbx5-/-) were compared to three clones (Het1, Het32, Het35) of BMEL Het cells (Cbx1+/+; Cbx3+/+; Cbx5+/-) as controls.

2026-03-31 | E-MTAB-15197 | biostudies-arrayexpress

RNAseq of BMEL cell lines invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)

Project description:Expression profiling by high throughput sequencing in Bi-potential Murine Embryonic Liver cells (BMEL cells). Three clones (TKO1, TKO2, TKO3) of BMEL TKO cells invalidated for the expression of the three HP1 isoforms (Cbx1-/-; Cbx3-/-; Cbx5-/-), compared to three clones (Het1, Het32, Het35) of BMEL Het cells (Cbx1+/+; Cbx3+/+; Cbx5+/-) expressing HP1 as controls. Expression profiling was performed on total cell extracts, on cytosolic RNA and on chromatin-enriched RNA.

2026-03-31 | E-MTAB-15194 | biostudies-arrayexpress

HP1, H3-K27me3, and H3-K9me3 Cut & Tag of Het (Cbx1+/+, Cbx3+/+, Cbx5+/-) and TKO (Cbx1-/-, Cbx3-/-, Cbx5-/-) bipotential mouse embryonic liver (BMEL) cells

Project description:HP1, H3-K27me3, and H3-K9me3 Cut & Tag of Het (Cbx1+/+, Cbx3+/+, Cbx5+/-) and TKO (Cbx1-/-, Cbx3-/-, Cbx5-/-) bipotential mouse embryonic liver (BMEL) cells

| PRJEB90041 | ENA

ATACseq in BMEL cells invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)

Project description:ATACseq in BMEL cells invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)

| PRJEB90156 | ENA

The HP1 protein interactome of mouse embryonic stem cells

Project description:The family of Heterochromatin Protein 1 (HP1) consists of highly conserved proteins, which have important functions in the nucleus of eukaryotic cells. In mammals there are three HP1 paralogs: HP1(alpha), Hp1(beta), and Hp1(gamma)They are encoded by the Cbx5, Cbx1, and Cbx3 genes, respectively. Hp1 and Hp1 stably interact with Chd4 and Adnp to form the ChAHP complex. In this project, Chd4, Adnp, and the three Cbx genes were endogenously tagged with a FLAG-Avi tag in mouse embryonic stem cells. The tagged proteins were subjected to tandem-affinity purification and analysis by mass spectrometry.

2018-05-22 | PXD006226 | Pride

RNAseq of BMEL cell lines invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)

Project description:RNAseq of BMEL cell lines invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)

| PRJEB90130 | ENA

ChIPseq of RNA Exosome components in BMEL cell lines invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)

Project description:ChIPseq of RNA Exosome components in BMEL cell lines invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)

| PRJEB90155 | ENA

Effect of depletion of Hp1α (Cbx5) and Hp1β (Cbx1) on gene expression of mouse embryonic stem cells (mESCs)

Project description:Effect of depletion of Hp1α (Cbx5) and Hp1β (Cbx1) on gene expression of mouse embryonic stem cells (mESCs)

| PRJNA833445 | ENA

ChIP-seq in SUNE1 cells that overexpressed CBX1

Project description:ChIP experiment against anti-FLAG in SUNE1 cells trasfected with FLAG-CBX1 plasmid.

2023-01-04 | GSE201232 | GEO