Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HP1, H3-K27me3, and H3-K9me3 Cut & Tag of Het (Cbx1+/+, Cbx3+/+, Cbx5+/-) and TKO (Cbx1-/-, Cbx3-/-, Cbx5-/-) bipotential mouse embryonic liver (BMEL) cells

ABSTRACT: HP1, H3-K27me3, and H3-K9me3 Cut & Tag of Het (Cbx1+/+, Cbx3+/+, Cbx5+/-) and TKO (Cbx1-/-, Cbx3-/-, Cbx5-/-) bipotential mouse embryonic liver (BMEL) cells

PROVIDER: PRJEB90041 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR15088104_1.fastq.gz	Fastqsanger.gz
	ERR15088104_2.fastq.gz	Fastqsanger.gz
	ERR15088105_1.fastq.gz	Fastqsanger.gz
	ERR15088105_2.fastq.gz	Fastqsanger.gz
	ERR15088106_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

The HP1 protein interactome of mouse embryonic stem cells

Project description:The family of Heterochromatin Protein 1 (HP1) consists of highly conserved proteins, which have important functions in the nucleus of eukaryotic cells. In mammals there are three HP1 paralogs: HP1(alpha), Hp1(beta), and Hp1(gamma)They are encoded by the Cbx5, Cbx1, and Cbx3 genes, respectively. Hp1 and Hp1 stably interact with Chd4 and Adnp to form the ChAHP complex. In this project, Chd4, Adnp, and the three Cbx genes were endogenously tagged with a FLAG-Avi tag in mouse embryonic stem cells. The tagged proteins were subjected to tandem-affinity purification and analysis by mass spectrometry.

2018-05-22 | PXD006226 | Pride

Effect of depletion of Hp1α (Cbx5) and Hp1β (Cbx1) on gene expression of mouse embryonic stem cells (mESCs)

Project description:Effect of depletion of Hp1α (Cbx5) and Hp1β (Cbx1) on gene expression of mouse embryonic stem cells (mESCs)

| PRJNA833445 | ENA

Cbx3 Maintains Lineage Specificity During Neural Differentiation [RNA-seq]

Project description:Cbx3 (HP1γ) that is a member of the heterochromatin protein 1 family play important roles in development and differentiation. To determine the regulatroy mechanisms of Cbx3 during neural differentiation from ESCs to NPCs, we performed RNA-seq analysis of ESCs or ESC-derived NPCs depleted for Cbx3 or Cbx3-assocatied Mediator subunit Med26.

2017-03-27 | GSE89574 | GEO

ChIP-seq in SUNE1 cells that overexpressed CBX1

Project description:ChIP experiment against anti-FLAG in SUNE1 cells trasfected with FLAG-CBX1 plasmid.

2023-01-04 | GSE201232 | GEO

Cbx3 Maintains Lineage Specificity During Neural Differentiation [ChIP-seq]

Project description:Cbx3 (HP1γ) that is a member of the heterochromatin protein 1 familiy enriched in gene bodies of pluripotent ESCs but promoters of differentiated pre-iPSCs. To determine whether Cbx3 becomes enriched at promoters to influence gene regulation during differentiation, we converted ESCs to NPCs in monolayer culuture and performed genomewide ChIP-seq of Cbx3. Our results shows that Cbx3 enriches at the promoters of genes upon differentiation of ESCs to NPCs and stablizes the binding of Mediator subunit Med26 to pre-initiation complex (PIC) in NPCs.

2017-03-27 | GSE89573 | GEO

Genome-wide maps of Cbx3 in pluripotent mESC and pre-iPSCs

Project description:We examined the locations of Cbx3 by chromatin immunoprecipitation in ESCs and pre-iPSCs Examination of Cbx3 in mESC (mouse embryonic stem cells) and pre-iPSCs (fibroblast derived partially reprogrammed cells)

2013-06-05 | E-GEOD-44242 | biostudies-arrayexpress

BRD4 represses CBX5

Project description:BRD4 is amplified and/or up-regiulated in a subset of ovarian cancer which correlates with a poor survival siRNA sensitization screens in the presence of CHK1 inhibitor, LY2606368, identify BRD4 as a therapeutic target in ovarian cancer. BRD4 suppression by either siRNA or using JQ1 increases CBX5 expression

2016-05-01 | GSE78704 | GEO

HP1β has distinct subnuclear localizations, chromatin binding features and functions in embryonic stem cells and differentiating cells [expression]

Project description:We used microarrays to identify the gene expression changes in Cbx1-/- (HP1beta) knockout embryonic stem cells (ESCs) and Cbx5-/- (HP1alpha) knockout ESCs compared to WT ESCs and in embryoid bodies (EBs) differentiated from those three ESC types.

2015-08-02 | GSE65121 | GEO

HP1γ/CBX3 Promotes Lung Adenocarcinoma by Activating Rho GTPases Signaling Pathway

Project description:We performed CBX3 knockdown in lung adenocarcinoma cells along with RNA sequencing. The expression profiling showed Rho GTPases signaling as a potential CBX3 target and the conclusions were also verified in clinical samples.

2021-05-05 | GSE173858 | GEO

Transcriptome analysis of the CBX3-depleted 143B osteosarcoma cell line.

Project description:To investigate the role of CBX3 in the progression of osteosarcoma, we performed a transcriptome analysis of the CBX3-depleted 143B cell line and a matched normal 143B cell line.Differential gene enrichment revealed activation of the anti-DNA damage pathway and activation of the apoptotic and P53 pathways.

2023-03-01 | GSE224201 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data