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HP1, H3-K27me3, and H3-K9me3 Cut & Tag of Het (Cbx1+/+, Cbx3+/+, Cbx5+/-) and TKO (Cbx1-/-, Cbx3-/-, Cbx5-/-) bipotential mouse embryonic liver (BMEL) cells

ABSTRACT: HP1, H3-K27me3, and H3-K9me3 Cut & Tag of Het (Cbx1+/+, Cbx3+/+, Cbx5+/-) and TKO (Cbx1-/-, Cbx3-/-, Cbx5-/-) bipotential mouse embryonic liver (BMEL) cells

PROVIDER: PRJEB90041 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR15088104_1.fastq.gz	Fastqsanger.gz
	ERR15088104_2.fastq.gz	Fastqsanger.gz
	ERR15088105_1.fastq.gz	Fastqsanger.gz
	ERR15088105_2.fastq.gz	Fastqsanger.gz
	ERR15088106_1.fastq.gz	Fastqsanger.gz

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HP1, H3-K27me3, and H3-K9me3 Cut & Tag of Het (Cbx1+/+, Cbx3+/+, Cbx5+/-) and TKO (Cbx1-/-, Cbx3-/-, Cbx5-/-) bipotential mouse embryonic liver (BMEL) cells

Project description:Cut & Tag was used to localize the 3 mouse HP1 proteins (Cbx1,Cbx3,Cbx5) to the genome of BMEL (bipotential mouse embryonic liver) cells. Het BMEL cells are Cbx1+/+, Cbx3+/+, Cbx5+/- HP1-TKO (triple knock-out cells) are Cbx1-/-, Cbx3-/-, Cbx5-/-. The HP1_TKO cells were used to verify the specificity of the Cut & Tag signals. HP1 localization was compared with the chromatin localization of select RNA exosome subunits and adapters to support a role for HP1 in targeting the RNA exosome to specific chromatin regions.

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ChIPseq of RNA Exosome components in BMEL cell lines invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)

Project description:Chromatin association of several nuclear RNA exosome components and cofactors in BMEL cells is dependent on the presence of HP1 proteins. TKO2 cells invalidated for the expression of the three HP1 isoforms (Cbx1-/-; Cbx3-/-; Cbx5-/-) are compared to Het32 cells (Cbx1+/+; Cbx3+/+; Cbx5+/-) expressing HP1 as controls.

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