Expression analysis of Lrig1-eGFP+ve and -ve cells from small intestine
Ontology highlight
ABSTRACT: Expression profiling array of Lrig1 expressing and non-expressing cells isolated by flow cytometry from the intestinal epithelium of Lrig1:eGFPiresCreERT2 (Lrig1 KI) animals.
Project description:Long-term tissue homeostasis is governed by balanced contribution from adult stem cells. We recently identified Lrig1 as a marker of stem cells in mouse epidermis. Here, we show that Lrig1 expressing cells are molecularly and functionally distinct from previously characterized epidermal stem cell populations. During steady state homeostasis, Lrig1 expressing cells are responsible for the maintenance of the uppermost compartment of the pilosebaceous unit known as the infundibulum. Lineage tracing demonstrates that the epidermis is divided into discrete compartments during homeostasis, each maintained by its own resident stem cells. Compartment boundaries are rapidly broken when stem cell progeny are recruited to sites of injury, where they subsequently change behavior according to the environment. Oncogene activation in Lrig1 expressing cells alters proliferation but auxiliary stimuli are required for rapid tumor growth. Our data demonstrate that stem cell niches are compartmentalized according to altering requirements for tissue replenishment.
Project description:Primary intestinal epithelial cells were isolated from the proximal part of the small intestine from either E16.5 foetal tissue or adult tissue and embedded in matrigel for culturing in in advanced F12/DMEM supplemented with EGF, R-spondin and Noggin. RNA was extracted from cultures established from independent animals and subjected to expression profiling.
Project description:Transcriptional profiling of hESCs in chemically-defined culture media compared to hESCs differentiated for 36h in the additional presence of FGF, LY294002 and either BMP or ActivinA.
Project description:Genome-wide expression analysis was performed with the aim of investigating transcriptional changes mediated by the transcription factor B lymphocyte-induced maturation protein-1 (Blimp1) in the context of pluripotent embryonic cells, with the aim of drawing parallels to its functions in mouse primordial germ cells.
Project description:Gene expression profiling experiment to identify genes up and down regulated by the presence of Activin during pancreatic specification.
Project description:Transcriptional profiling comparison of mouse embryonic stem cells versus their JAK mutated counterparts, cultured both in the presence and absence of LIF (leukemia inhibitory factor).
Project description:Transcriptional profiling of H9 human embryonic stem cells differentiated towards early endoderm over 72 hours in chemically defined media.
Project description:Transcriptional profiling of hESCs differentiated towards an endodermal fate in chemically-defined media (3 days) compared to clones subjected to EOMES knockdown (via shRNA) under the same conditions.