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Bulk-RNA of MACS isolated Microglia from a Parkinson Mouse Model brain from young and old mice and Parkinson and Control

ABSTRACT: Microglia are increasingly recognized as active drivers of Parkinson's disease (PD), contributing to neuroinflammation, α-synuclein clearance, and dopaminergic neuron loss. Because aging is the strongest risk factor for PD and independently shifts microglia toward a primed, pro-inflammatory state, disentangling age- and disease-related transcriptional programs is essential. Aim and workflow This dataset profiles the bulk transcriptome of microglia from [PD model] and control mice at [young] and [old] ages (2×2 design) to resolve disease, age, and interaction effects. Brains were enzymatically dissociated, microglia isolated by MACS using CD11b MicroBeads, and total RNA extracted for bulk RNA-seq library preparation and sequencing on Novaseq 6000. n = 3-4 per group. Both sexes included. All samples except Sample1 includes two mice. Sequencing was done with a target of 40G per sample PE150.

INSTRUMENT(S): --, Illumina NovaSeq X

ORGANISM(S): Mus musculus

SUBMITTER: Jaehyun Lee

PROVIDER: E-MTAB-17136 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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