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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single-nucleus RNA-seq of FACS-enriched Glp1r-Sun1-sfGFP nuclei from mouse hypothalamus, dorsal vagal complex, and nodose ganglion

ABSTRACT: Glucagon-like peptide-1 receptor (GLP1R) agonists such as semaglutide reduce food intake and body weight, and Glp1r-expressing neurons in the hypothalamus and hindbrain mediate these effects. To profile these cells directly, Glp1r-Cre mice were crossed to the Gt(ROSA)26Sor-CAG-Sun1-sfGFP reporter, which labels the nuclear envelope of Glp1r-expressing cells with GFP. The hypothalamus, dorsal vagal complex, and nodose ganglion were dissected and flash-frozen; tissue was pooled from two or more mice of both sexes per library. Nuclei were isolated, stained with propidium iodide, and GFP+/PI+ nuclei collected by fluorescence-activated sorting. Libraries were prepared using the 10x Genomics Chromium Single Cell 3' v3 platform, targeting approximately 10,000 recovered nuclei per library, and sequenced on an Illumina NovaSeq 6000 as 151 bp paired-end reads. Reads were mapped with Cell Ranger 6.0.0 to the mm10-2020-A reference. Each library contains nuclei from all three regions; region of origin was assigned informatically rather than by separate library preparation. Animals received semaglutide or vehicle, indicated per sample, though treatment was not a variable in the reported analysis, which used the data to identify cell-type marker genes.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Brian Gural

PROVIDER: E-MTAB-17566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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