Project description:Evaluate differences in gene methylation levels between obese men with and without the metabolic syndrome Visceral adipose tissue from obese men with the metabolic syndrome (MetS+, N=7) vs. obese men without the metabolic syndrome (MetS-, N=7)
Project description:Adipose tissue before and after bariatric surgery (BPD/DS)-Pilot study using AB1700 microarrays. Subcutaneous abdominal adipose tissue pre and post bariatric surgery (BPD/DS).
Project description:Identification of CpG sites associated to plasma TG levels Bisulphite converted DNA from 24 visceral adipose tissue (VAT) samples were hybridised to the Illumina Infinium HumanMethylation450 Beadchip. Contributor: the Multiple Tissue Human Expression Resource Consortium
Project description:To find out the regulating pathways of AIP1 in Type 2 Diabetes, we performed a proteomic array of omental adipose tissue between obese T2D and obese patients.
Project description:RNA-seq files collected from an in vitro differentiation time course of primary human adipocytes from lean, obese, and obese with T2D individuals. RNA-seq data was collected at confluence (day 0), day 3, and day 15 of differentiation. \"Conf\" samples are preadipocytes, \"3days\" and \"15days\" are differentiated adipocytes.
Project description:Comparison of subcutaneous abdominal adipose tissue before and after biliopancreatic diversion with duodenal switch (BPD/DS), and versus subcutaneous abdominal adipose tissue from lean, healthy subjects undergoing hernia repair surgery
Project description:Genes involved in the autophagic pathway are risk factors for the development of inflammatory bowel disease. In addition, patients with Crohn’s disease exhibit a profound expansion of mesenteric fat during the pathology. We investigated in our study the role of autophagy in mature adipocyte during intestinal inflammation. Mice bearing a tamoxifen-inducible deletion of Atg7 specifically in adipocytes showed signs of exacerbated intestinal inflammation in response to DSS-induced colitis. We therefore aimed to understand the possible underlying reasons for this change and performed a bulk RNA-seq approach using primary visceral adipocytes.
Project description:Obesity, a major risk factor for chronic diseases, is related to dsyfunctional adipose tissue signaling. First human trials suggest benefits of intermittent calorie restriction diet (ICR) in chronic disease prevention that may exceed those of continuous calorie restriction diet (CCR), even at equal net calorie intake. The effect of intermittent calorie restriction on adipose tissue signaling has not been investigated to date. Thus we initiated a randomized controlled trial to analyze the effect of ICR (eu-caloric diet on five days and two days per week with energy restriction of 75%), CCR (daily energy restriction of 20%) and a control group on subcutaneous adipose tissue (SAT) gene expression. 150 overweight or obese non-smoking adults (50 per group, 50% women) were randomly asiged to one of the study arms. SAT biopsies were taken before and after the 12 week intervention phase.
Project description:Brown adipose tissue (BAT) regulates whole-body energy balance through UCP1-dependent thermogenesis and secretion of metabolic factors. Our single-nucleus RNA sequencing of BAT from cold-exposed Ucp1 knockout mice reveals a distinct brown adipocyte subpopulation (U2). U2 adipocytes exhibit a secretory profile enriched in batokines like growth differentiation factor 15 (GDF15), suggesting a shift towards an endocrine role. Functional analyses reveal that GDF15-GFRAL signaling is required to sustain energy expenditure in adipose tissue (AT). Additionally, a conserved UCP1-GDF15 regulatory axis in human AT is observed.